Having overcome Chorley in the first round on Saturday, The Lions will play away at Nuneaton Town in the second round of the FA Trophy.
The trip to the National League North side is scheduled to take place on Saturday 14th January, with a 3pm kick off.
Nuneaton were relegated after a three year spell in the National League in 2014-15, the same season Guiseley won promotion. The Boro had previously beaten Guiseley in extra time in the play off semi final on their way to promotion in 2012.