Defensive frailties once again proved to be the Lions undoing as goals from Craig Dundas and Tom Bolarinwa sent Sutton top of the pile while condemning Guiseley to second from bottom.
In between United goals Lee Molyneux had the chance to square matters but Jamie Butler was equal to his spot kick given for a handball in the area.
Sutton took the lead on 12 minutes after a Kieron Cadogan corner was not cleared and headed back into the danger area by Jamie Collins for Dundas to pounce.
To compound matters further, within a couple of minutes of Molyneux’s penalty miss Tom Bolarinwa raced in ahead of Jonny Maxted to prod Cadogan’s floated ball home and double the visitors lead.
Andy Howarth and Raul Correia replaced Molyneux on the restart which saw the home side increase the tempo but even then the closest they came was when Howarth’s drive was tipped on to then post by Butler late on.
But overall it was a question of too little too late as Sutton returned south with all three points leaving manager Paul Cox with plenty to ponder ahead of the Lions historic Emirates FA Cup Second Round tie at Mansfield a week tomorrow.
11 Molyneux (Haworth 46)
28 Lenighan (Rooney 75)
19 Odejayi (Correia 46)