Mark Bower’s Lions host Championship side Barnsley on Tuesday night with kick-off at 7:30pm.
The Tykes enjoyed a successful season last time out securing promotion and the Johnstone’s Paint Trophy at Wembley.
Paul Heckingbottom was appointed the Barnsley manager last month after a spell in charge as caretaker boss at the end of last season.
The South Yorkshire side are expected to have new signings and trialists on show including George Moncur and Elliott Lee.
Also faces familiar to Guiseley fans Kayden Jackson and Stefan Payne who signed from Wrexham and Dover respectively should feature on Tuesday night.
Bower has hinted that the number of trialists will be reduced for this fixture as he looks to make additions to his squad for next season.
“We have seen a bit of them now they’ve seen a bit of us, some players will have options to go elsewhere.”
“The lads that have come in have given us some real food for thought, we will have to have a chat between myself, Martin and Bosh as to how many stay on for a bit longer or who we look to offer deals to and those who move on.”
Mark also stated the impact Oli Johnson could have next season if he stays injury free.
“Having him fit will be a massive massive bonus for us, we know coming off the bench or when he starts he has the quality to turn a game for us.”
“We have got to make sure he is well looked after, he is doing a lot of work all by himself and is really keen to make sure he is right for next season.”
“If there’s one player you really feel for from a personal point of view that you want to succeed and have an injury free season it is Oli Johnson.”
“For him as a person I really hope he can stay fit and enjoy his football this season.”
Looking ahead to the third home pre-season fixture the Lions boss is looking to make changes to his playing staff and tactics.
“It will test our fitness, I might try one or two different things, a couple of different faces will be in on Tuesday to find people’s strengths and weaknesses and how they adapt in different formations.”
Remember there’s free admission for Guiseley season ticket holders and tickets for the upcoming friendly with Leeds United will also be on sale in the clubhouse pre-match.